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文献和实验Characterization of Epithelial Cells in the Hair Follicle With S100 Proteins
proteins are expressed in very restricted regions of the hair follicle: S100A3 and S100A6 in distinct postmitotic differentiated epithelial cells and S100A4 and S100A6 in the epithelial stem cell compartments. Characterization of epithelial cells
Corneal Injury: A Relatively Pure Model of Stromal-Epithelial Interactions in Wound Healing
Wound healing is a complex cascade that varies depending on the organ being studied. However, many components of the response are similar in different organs. Stromal-epithelial interactions are critical to wound healing in tissues
操作步骤参考[3] 1. 收集:100g 离心 5min 收集类器官。 2. 重悬:收集到的类器官重悬于 1× PBS 中,转移至 96 孔圆底 (U 型) 板中,100g 离心 5min。 3. 固定:弃上清,加入 4% 多聚甲醛室温固定 30min。 4. 洗涤:移除多聚甲醛,用 1× PBS 洗涤 3 次,每次 100g 离心 5min。 5. 透化:加入 0.3% Triton-X,室温透化 10min。 6. 封闭:加入含有 10% 驴血清的 PBST
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