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文献和实验RNA Purification Protocol for 10-20mg of Animal Tissue
# SSI-1015-39). 3. Add 200 μl of PNP Buffer to the cell lysate. Mix the sample gently by inverting the tube 10 times. 4. Place the tube on ice for 5 minutes. 5. Centrifuge at max speed (.13,000 x g) for 3 minutes at room temperature
GENERAL MAINTENANCE INSTRUCTIONS FOR IMAGINAL DISC CELL LINES
The complete M3 medium has the following additives. 2% FBS 2.5% fly extract 5 ug/ml insulin 1/2 x penstrep (100 x bottle = 5000 ug/ml) Adding insulin. Insulin (Sigma, I-6634) Dissolve 5mg insulin in 0.3ml of 0.01N HCl each time
with PI: 1. Harvest cells and prepare single cell suspension in buffer (e.g. PBS + 2% FBS; PBS + 0.1% BSA) 2. Wash cells X2 and resuspend at 1-2 x 106 cells/ml. 3. Aliquot 1 ml cells in a 15 ml polypropylene, V-bottomed tube and add 3 ml cold
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