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文献和实验40, then again with PBS/0.1 BSA. Mount solution (70 glycerol containing 2 n-propyl gallate and 0.25 ug/ml Hoechst) is placed on the cells. A coverslip is placed and sealed with nail polish. Slides are stored at -20oC until examination. Antibodies mouse
containing 0.1% NP40, then again with PBS/0.1% BSA. Mount solution (70% glycerol containing 2% n-propyl gallate and 0.25 ug/ml Hoechst) is placed on the cells. A coverslip is placed and sealed with nail polish. Slides are stored at -20o C
RNA Purification Protocol for 10-20mg of Animal Tissue
设备 1. Microcentrifuge capable of 13,000 x g 2. Sterile 1.5 mL microcentrifuge tubes 3. Ice bath 4. Microfuge tube pestle 实验步骤 1. Dissect tissue quickly and freeze in liquid nitrogen. Storage at -70°C
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