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- 文献和实验
- 技术资料
- 英文名:
Os 1000 µg/mL in 10% HCl
- 库存:
1
- 供应商:
甄准生物
- 规格:
100ML
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文献和实验(note 7).Materials & Reagents ChIP lysis buffer 30ml final volume: 1.5 ml 1M Hepes 1.05ml 4M NaCl 3ml 10% Triton X (Tx-100) 300µl 10% NAD 24.15ml H20 ChIP high salt buffer 30ml final volume: 1.5ml 1M Hepes
1.5M Tris(PH 8.8) 100ml 18.171g (at4°C) 10% SDS 100 ml 10g (at room temperature) 10% ammonium persulfate 1ml 0.1g(replace every week store at at4°C) TEMED 8 room temprature Antibodies Primary antibody: 5A11(anti-mouse IgG) 500x-1000x Secondary
Rapid Extraction of High Quality DNA from Whole Blood Stored at 4ºC for Long Period
should be shake gently by rotating blood mixer (vortex) Pour 500 µl of blood into a 1.5 ml eppendorf tube and add 1000 µl of red cell lysis buffer. Shake microfuge tube gently (up to homogenizing), then spin for 2 minutes at 7000 rpm. Discard
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