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- Chemservice-X10005-1-250ML
- 2025年07月16日
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- 详细信息
- 文献和实验
- 技术资料
- 英文名：
Be 1000 µg/mL in 2% HNO3
- 库存：
1
- 供应商：
甄准生物
- 规格：
250ML
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文献和实验2. The next day, aliquot the 2 - 10 million cells per sample. Cells in 1 ml of the 2% FCS, DMEM, transfer to an Eppendorf tube and stimulate with agonists of choice, for the required time and temperature. 3. Spin for 5 - 10 min @ 1000x g at room temperature
The ribonuclease protection assay (RPA)
% acrylamide 9.31 mls of 2% bis acrylamide 7.45 mls of 10x TBE 35.82 g of Urea QS to 74.5 ml with dH2O 450 µl ammonium persulfate (10%) 60 µl TEMED Pour immediately into the gel mold, remove any air bubbles, and add an appropriate comb (e.g
Native Chromatin Preparation and Illumina/Solexa Library Construction
Protease inhibitor cocktail for ChIP (1000X) Proteinase K (20 mg/mL) (Roche Diagnostics) Reagents for agarose gel electrophoresis RIPA buffer for ChIP RNase A SDS (sodium dodecyl sulfate) (10%) T cell isolation buffer
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