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- 库存：
1 case
- 供应商：
西宝生物
- 保存条件：
ROOM TEMPERATURE
- 英文名：
260ML PS FLASK, STR.NECK W/VENT
- 规格：
LABWARE
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文献和实验Construction and Characterization of Adenovirus Vectors
as described above or in suspension. Grow the cells in suspension in 1X maintenance medium in suspension flasks, agitated at 70 rpm. When they reach a density of 5 x 105 cells/mL, dilute 1:2 or 1:3 with 1X maintenance medium. CsCl (cesium chloride
. Incubate for 15 min at 37 °C. Rinse each flask x 2 with 10 ml DPBS (without Ca or Mg). 6) Add 4 ml of Trypsin to each flask. Incubate at 37 °C for 3 min. Neutralize with 8 ml of Trypsin Neutralization Solution. 7) Wash
media. 6 3. Insert the tip of a sterile pipette into the stock bottle and remove 10 ml of media. Transfer the media to the tissue culture flask. 4. Open the top of the suspension culture and use a sterile 1.0 ml transfer pipette to remove a 1.0 ml
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