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- 详细信息
- 文献和实验
- 技术资料
- 库存：
50/cs
- 供应商：
西宝生物
- 英文名：
600ML PS FLASK, ANG.NECK W/FILTER CAP
- 保存条件：
ROOM TEMPERATURE
- 规格：
LABWARE
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文献和实验250 ml of SOB. The first flask receives 10 ml of starter culture, the second receives 4 ml, and the third receives 2 ml. Incubate all three flasks overnight at 18-22ºC with moderate shaking. The following morning, read the OD600
. Incubate for 15 min at 37 °C. Rinse each flask x 2 with 10 ml DPBS (without Ca or Mg). 6) Add 4 ml of Trypsin to each flask. Incubate at 37 °C for 3 min. Neutralize with 8 ml of Trypsin Neutralization Solution. 7) Wash
culture hood. Ensure that any alcohols and wrapping paper are kept clear of the bunsen burner. Pre-sterilize the hood before use, and use disposable sterile gloves. Loosen the cap of a tissue culture flask and the cap of a stock bottle of tissue culture
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