Cullin-Associated NEDD8-Dissoc

Analysis of Protein-DNA Binding by Streptavidin-Agarose Pulldown

and an array of associated proteins that interact with the basal transcription factors, thereby activating the transcription machinery. Analysis of the large complex of proteins that bind to DNA is an important step in elucidating the mechanisms by which gene

神奇的模式植物--拟南芥

对生长素响应的降低[3]。随后在动物中鉴定了RUB1的同源物Nedd8，研究表明Nedd8同样是SCF复合体发挥功能所必需的[4]，而SCF功能紊乱与多种人类疾病如癌症、阿尔兹海默症等密切相关[5]。由此可见，拟南芥中生长素信号途径的研究对于认识人类某些疾病的发病机理提供了重要帮助。 模式植物的选择和利用对于开展遗传分析、基因克隆和功能研究意义重大，拟南芥由于其植株小、结实多、生命周期短、基因组简单、遗传操作简便，近四十年来由田野里不起眼的小草成为植物研究领域最耀眼的明星。全世界

Agonist-Induced High-Affinity GTP Hydrolysis as an Index of Receptor-Mediated G Protein Activation in Mammalian Brain Membranes

-mediated signal transduction have not yet been fully elucidated, the following scheme is generally accepted (Fig. 1 ). In an inactive state, G protein exists as an associated form of GDP-bound a-subunit and fiy-subunits (Fig. 1A ). When an agonist is bound