BIA 10-2474,BIA 10-2474,123385

生物反应过程培养液成分在线检测技术

)，A为参与晶振的面积(cm2 )。压电生物传感技术得到迅速发展，应用广泛。Su等在石英晶体表面固定DNA，用于检测抗癌铂药物的含量，检测限为l0-7 mol/L[10] ；Liu等使用串联电极压电晶体传感器对L-谷氨酸进行检测，检测限为3.8×lO-5 mol/L[11] 。 2.2 核磁共振(NMR) 如同紫外线或红外线的吸收会产生电子跃迁一样，吸收适当频率的电磁辐射线也能引起由强磁场产生的原子核磁级的跃迁。分子环境影响磁场中原子核的吸收，影响因素与分子结构有关。测量

Two‐Step, One‐Pot Synthesis of Inosine, Guanosine, and 2′‐Deoxyguanosine O6‐Ethers via Intermediate O6‐(Benzotriazol‐1‐yl) Derivatives

) and Cs2 CO3 under a nitrogen atmosphere. Conversion to the O 6 ?(benzotriazol?1?yl) ethers occurs within about 10 min for inosine, and within about 60 min for guanosine and 2??deoxyguanosine. Then, for reaction with alcohols, the reaction mixture