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- 详细信息
- 文献和实验
- 技术资料
- 英文名：
5,6,7,8-Tetrahydro-benzo4,5thieno2,3-dpyrimidin-4-ylamine
- 库存：
999
- 供应商：
智溯科ZSK
- CAS号：
4994-88-1
- 规格：
10mg///100mg///200mg
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文献和实验PRODUCTION OF COMPLETELY ES CELL-DERIVED FETUSES BY AGGREGATION WITH TETRAPLOID
The technique described here is a slight modification (March 1997) of methods presented in: Nagy A., J. Rossant. 1993. Production of completely ES cell-derived fetuses. In : Gene Targeting: A Practical Approach. (ed. A. Joyner). IRL Press at Oxford
in 37°C water bath for 10 minutes. 6) Centrifuge on setting 4 for 5 minutes. 7) Aspirate supernatant and flick pellet. In the next step, be careful to disperse the cells thoroughly in the fix. Add fresh fix (2 ml glacial acetic acid and 5 ml
An actively growing culture of cells is required, i e 2 - 3 d ES cell culture. The total number of cells needs to be between 106 - 107 cells. N B Read notes at end of method. 1) Harvest cells in the usual manner, into a conical
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