重组人 EGFR蛋白 (PE定点偶联, Isoform vIII, His 标签)

EGFR重组蛋白|Recombinant Human EGFR Protein (Site-Specific PE Conjugation, Isoform vIII, His Tag)（产品说明）
蛋白名称：Human EGFR Protein (Site-Specific PE Conjugation, Isoform vIII, His Tag)A DNA sequence encoding the human EGFR (NP_001333870.1, Isoform vIII) (Met1-Ser378) was expressed with a C-terminal polyhistidine tag. The protein is site-specifically conjugated with PE (Excitation= 495 - 566 nm, Emission Max.= 575 nm).
表达宿主：HEK293 Cells
蛋白纯度：1.Flow cytometric analysis of anti-EGFR CAR expression. 5e5 of anti-EGFR CAR-293 cells were stained with 100 μL of 1:100 (1 μL stock solution in 100 μL FACS buffer) of PE-conjugated human EGFRvIII Protein (Site-Specific PE Conjugation, Isoform vIII, His Tag, Cat. No. 29662-H86H-SP) and negative control protein. Non-transduced 293 cells were used as a control (left). PE signal was used to evaluate the binding activity (QC tested). 2.Binding activity of PE-conjugated EGFRvIII protein to PBMC cells. PBMC cells were stained with FITC conjugated anti-CD3 antibody and PE-conjugated human EGFRvIII Protein (Site-Specific PE Conjugation, Isoform vIII, His Tag, Cat. No. 29662-H86H-SP) and detected by flow cytometry. FITC signal was used to evaluate the content of CD3+ T cells in PBMCs. PE signal was used to evaluate the non-specific binding activity to PBMCs (QC tested).< 1.0 EU per μg protein as determined by the LAL method.Leu 25The protein has a predicted molecular mass of 42 kDa.NP_001333870.1 & Q59FL8Met1-Ser378