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- 文献和实验
- 技术资料
- 英文名:
D6-2,3-Butane-diol (mixture of stereoisomers)
- 库存:
999
- 供应商:
智溯科ZSK
- CAS号:
labelled:344750-80-7;unlabelled:513-85-9
- 规格:
1mg///5mg///10mg
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文献和实验Fast and reliable mini-prep RNA extraction from Neurospora crassa
otherwise. - Pulverize the mycelium in a mortar with liquid nitrogen - Transfer the powder into a mixture of 0.75 ml lysis buffer (0.6 M NaCl, 10 mM EDTA, 100 mM Tris HCl, pH 8.0, 4 SDS) and 0.75 ml phenol (saturated with 0.1 M Tris HCl, pH 8.0) in a 2 ml
! Annealing[Annealing: Spontaneous alignment of two single DNA strands to form a double helix.]: The reaction mixture is cooled down.Primers anneal to the complementary regions in the DNA template [Template: A molecule that serves as the pattern
Generation of uni-directional nested deletions in plasmid DNA
. 2) Heat inactivate restriction enzymes at 85℃for 30 mins. 3) Phenol/chloroform, chloroform extract and alcohol precipitate digested DNA. 4) Redissolve DNA in 20μl 1x One Phor All buffer. 5) Add 20μl 2x ExoIII buffer and preincubate the mixture
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