氘8-马波氟沙星盐酸盐 D8-Marbofloxacin
HCl labelled:1185053-37-5 free base;unlabelled:115551-26-3 **科研现货速达** ≥98% HPLC 随货提供COA、HPLC谱图、MS谱图，NMR图谱等，详细联系客服。用于LC-MS/MS定量分析，代谢组学、质谱，药代动力学等- 询价
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- 进口
- ISX-ST-7917
- 2025年07月13日
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- 详细信息
- 文献和实验
- 技术资料
- 英文名：
D8-Marbofloxacin HCl
- 库存：
999
- 供应商：
智溯科ZSK
- CAS号：
labelled:1185053-37-5 free base;unlabelled:115551-26-3
- 规格：
1mg///5mg///10mg
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文献和实验E.Z.N.A. Endo-Free BACs, PACs, P1s Protocol
实验步骤 1. Isolate a single colony from a freshly streaked selective plate, and inoculate a starter culture of 2-5 ml LB or YT medium containing the appropriate selective antibiotic. Incubate for ~ 20-24 hr at 37°C
PCR引物设计和优化(PCR PRIMER DESIGN AND REACTION OPTIMISATION)
with digoxygenin-11-dUTP （DIG; Roche） need be done only in 50uM each dNTP, with the dTTP substituted to 35% with DIG-11-dUTP. NOTE: that the product will have a higher MW than the native product! This results in a very well labelled probe which can be extensively
RNase Protection Assay (Beverly Faulkner-Jones)
is completely dissolved8 - heat at 37oC if necessary - before adding the probe 8 Add 1 ml of the cRNA probe which must be in excess for accurate quantitation. The amount has to be determined empirically but as a rough rule of thumb, using a 200 - 400 base probe
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