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- 详细信息
- 文献和实验
- 技术资料
- 英文名:
D11-4-Phenylbutyric Acid
- 库存:
999
- 供应商:
智溯科ZSK
- CAS号:
labelled:358730-86-6;unlabelled:1821-12-1
- 规格:
1mg///5mg///10mg
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文献和实验v468 Chapter 7 通过免疫组化来测定β-连环蛋白核定位
(Klinipath). 5. Cold plate (approximately –12°C). 6. Microtome. 7. Starfrost microscope slides. 8. Coverslips (Menzel-Gl?ser, Braunschweig, Germany). 2.3. Immunohistochemical Staining 1. Peroxidase (PO) blocking buffer: 0.040 M citric acid
Marcantonio Lab Protocol Manual
because they do not bind to the silica matrix, as well as removing any salts or ethidium bromide. 11) Spin the tube in a Speed-Vac concentrator to remove the last traces of NEW WASH. 12) Resuspend pellet in 25 ul of DD-H2O (or any other low salt buffer such as TE
STANDARD PLANT MOLECULAR BIOLOGY PROTOCOLS
phenol/chloroform/isoamyl alcohol (25:24:1), vortex, centrifuge for 5 min and remove the supernatant to new tubes 11. Repeat step 10 12. Repeat this step with chloroform alone 13. Add 300 l isopropanol and incubate on ice for 10 min 14
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