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- 文献和实验
- 技术资料
- 英文名:
D3-Ibuprofen Carboxylic Acid (Mixture of Diastereomers)
- 库存:
999
- 供应商:
智溯科ZSK
- CAS号:
labelled:1216505-29-1
- 规格:
1mg///5mg///10mg
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文献和实验glyoxal (deionized) Glyoxal is purchased as a 40% (6M) solution (e.g. from Sigma). Glyoxal readily oxidizes in air. Oxidation of the solution can be detected by the lowering of its pH as carboxylic acids accumulate. These charged acids can be removed
Northern Blotting方法(Howell Lab)
on ice for 5 min. (If it's a total of 10 ml, use 2 mg salmon sperm.) Add the hybridization buffer (5 ml) and the formamide (5 ml) to the salmon sperm. Put the membrane in a tube and wet it with this mixture. Incubate at 42℃
Marcantonio Lab Protocol Manual
solution and vortex to mix. Let stand on ice for 5 minutes. This neutralizes the mixture, causing the plasmid DNA to reanneal rapidly. Most of the chromosomal DNA and bacterial proteins precipitate along with the SDS-potassium complex. 7) Centrifuge
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