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- 详细信息
- 文献和实验
- 技术资料
- 英文名:
13C2-Dimethyl Phosphate Sodium Salt
- 库存:
999
- 供应商:
智溯科ZSK
- CAS号:
labelled:157487-95-1
- 规格:
1mg///5mg///10mg
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文献和实验Southern Hybridization Protocols
. Add monobasic sodium phosphate salt gradually to adjust pH to 7.2 while warming up the solution on a hot plate. Add gradually 35g SDS, 10g BSA and 0.185g EDTA. Finally top up buffer to 500 ml. Aliquot 12.5 ml/polypropylene tube
Growing feeder-independent embryonic stem cells§
, picking and expanding single colonies, and testing several clones for germline competence. The majority of BayGenomics cell lines are derived from the E14Tg2A.4 subclone. Tissue culture reagents Dulbecco's phosphate-buffered saline (PBS
M Tris pH 9.6 200 µl 1M MgCl2 500 µl 5 mg/ml BCIP in dimethyl formamide (BCIP= 5-bromo-4- chloro-3-indoyl phosphate, toluidine salt), US Biochemical). Store this stock at -20° wrapped in foil; it's light sensitive. 2.5 ml 2 mg/ml NBT (p-nitro blue
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