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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
冷冻-20℃
- 英文名:
N-arachidoyl-D-erythro-sphingosine
- 库存:
999+
- 供应商:
上海维亦特生物科技有限公司
- CAS号:
123482-93-9
别名:N-arachidoyl-D-erythro-sphingosine
分子式:C38H75NO3
分子量:594.007
稳定性:1 Year
存储:冷冻-20°C
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文献和实验pmoles of ceramide and diolein. 4) Dry down lipids using extra dry nitrogen gas. 5) Resuspend lipids in each tube in 20 µl of b-OG/DOPG (7.5%:25 mM mixed micelle) and vortex vigorously. ---> Have a blank tube containing b-OG/DOPG
1.2-1.5 ml of the lower phase (total volume of lower phase should constitute 2 ml, but to prevent any aqueous contamination only a portion is used). ---> Also aliquot standards: 10, 20, 40, 80 & 160 pmoles of ceramide and diolein. 4) Dry
siRNA (RNA Oligo) Annealing Protocol
3: 100 mM Potassium Acetate 30 mM HEPES-KOH at pH 7.4 2 mM Magnesium Acetate Note: All formulas can be used without much difference. All annealing solutions are given as 5X and can be stored frozen at -20ºC and freeze-thawed
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