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- 详细信息
- 文献和实验
- 技术资料
- 抗体名:
PE/Cyanine7 anti-human CD66b
- 抗体英文名:
PE/Cyanine7 anti-human CD66b
- 应用范围:
免疫荧光,流式
- 宿主:
小鼠
- 供应商:
上海善然生物
- 库存:
现货
- 抗原来源:
小鼠
- 适应物种:
Human Reported Reactivity Chimpanzee
- 标记物:
PE/Cyanine7
- 克隆性:
单克隆
- 保存条件:
2-8度
- 形态:
液体
- 规格:
100 tests
PE/Cyanine7 anti-human CD66b Antibody
G10F5
Catalog# / Size 305115 / 25 tests
305116 / 100 tests
Clone
Regulatory Status RUO
Workshop VI MA81
Other Names CD67, CGM6, NCA-95, CEACAM8
Isotype Mouse IgM, κ
Description CD66b is a 95-100 kD glycosylphosphatidylinositol (GPI)-linked protein also known as CD67,
CGM6, and NCA-95. CD66b is a member of the immunoglobulin superfamily,
carcinoembryonic antigen (CEA)-like subfamily. CD66b, expressed on granulocytes, has been
reported to induce activation in neutrophils and to be involved in heterophilic adhesion with
CD66c.
Product Details
Verified Reactivity Human
Reported Reactivity Chimpanzee
Antibody Type Monoclonal
Host Species Mouse
Formulation Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under
optimal conditions.
Concentration Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our
Certificate of Analysis online tool.)
Storage & Handling The antibody solution should be stored undiluted between 2°C and 8°C, and protected from
prolonged exposure to light. Do not freeze.
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文献和实验更多产品详细信息欢迎来电咨询~周祥15900853496(微信同号,汪海洋15901695283微信同号
、CD14-PE、CD19-PE、CD3-PE、CD66b-PE、CD56-PE-Cy7、CD11c-APC、 CD45-AF700、CD38-APC-AF750(每次测试 5μL),以及 CD16-FITC(每次测试 20 μL),并在冰上孵育 30 分钟。10. 在 4℃ 条件下,300 x G 离心细胞 5 分钟。11. 吸取上清液。12. 重悬到 500 μL 染色缓冲液中。13. 通过 CytoFLEX 获取结果。通过 CytoFLEX 进行数据采集:1. 创建新补偿值。2. 如适用
Phycoerythrin conjugation protocol
and Centricon concentrators. Add 0.5% sodium azide. Store at 4 °C protected from light. Do not freeze. Figure 1. Analysis of column eluate fractions from PE conjugation of monoclonal antibody IB4 (murine IgG2a, anti-human CD
Immunofluorescence Staining of Human Cells by Lysed Whole Blood Method
to each tube. Centrifuge for 5 minutes at 1000 rpm (200 x g). Remove supernatant by aspiration and vortex. If third step is not required, proceed to step 18. For third step, add Sav-PE to each tube and vortex gently. Repeat steps 11-15
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