- ￥3776
- BioLegend-HK
- 534904
- 美国
- 2025年07月14日
- 免疫荧光，流式
- 小鼠
- 人
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- 技术资料
- 抗体名：
Biotin anti-human PlGF
- 抗体英文名：
Biotin anti-human PlGF
- 浓度：
0.5 mg/mL
- 应用范围：
免疫荧光，流式
- 宿主：
小鼠
- 供应商：
上海善然生物
- 库存：
4-5周
- 抗原来源：
小鼠
- 保质期：
2-3年左右
- 适应物种：
人
- 标记物：
Biotin
- 克隆性：
单克隆
- 保存条件：
2-8度
- 形态：
液体
- 规格：
100 μg
Biotin anti-human PlGF Antibody
24G3
Catalog# / Size 534904 / 100 µg
Clone
Regulatory Status RUO
Other Names PlGF, PLGF, Placental Growth Factor
Isotype Mouse IgG2a
Description Placental growth factor (PlGF) is a member of the PDGF/VEGF family of growth factors. Four
alternatively-spliced isoforms have been identified: PlGF-1, PlGF-2, PlGF-3, and PlGF-4. All
isoforms are expressed in placental tissues, with PlGF-2 being expressed specifically during
early placental development. PlGF-1 and PlGF-3 are considered diffusible isoforms, while
PlGF-2 and PlGF-4 each contain a unique heparin binding domain that may increase
associations with cell membranes. In addition to placental tissues, PlGF is expressed in the
microvasculature, bone marrow, umbilical vein endothelia, uterine keratinocytes, and NK cells.
PlGF binds to both VEGF-R1 and neuropilin, but not VEGF-R2 and induces monocyte
activation, migration, inflammatory cytokine production. PlGF plays a key role in maternal
vascular function during pregnancy, with circulating levels reaching a peak mid-gestation. It
can be used as a marker for pregnancy-associated hypertensive disorders such as preeclampsia.
Product Details
Verified Reactivity Human
Antibody Type Monoclonal
Host Species Mouse
Immunogen Recombinant protein
Formulation Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation The antibody was purified by affinity chromatography and conjugated with biotin under optimal
conditions.
Concentration 0.5 mg/ml
Storage & Handling The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
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A number of endogenously biotinylated proteins are found in both cytosol and mitochondria of mammalian cells from many tissues, including liver, spleen, pancreas, kidney, and intestine. Therefore, caution should be taken when using the biotin
in the nucleus in human cell cultures we designed several RNA-biotin based pulldown assays which can be used alone or in combination with other known assays such as ChIP and Flag-tagged pulldown assays. Three protocols are explained
CGH of Biotin Labeled DNA vs Digoxigenin Labeled DNA
DAY 1: 1) Reprecipitate DNA's Add the following DNA's to a 1.5 ml centrifuge tube, mixing with pipet: 20 ug Cot-1 DNA (~20ul) ~200 ng Biotin labeled DNA (~10ul) ~200 ng Digoxigenin labeled DNA (~10ul) Add 1/10th
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