228-18837-91 岛津 Shim-pack 6.0M

高效液相色谱法测定心舒颗粒中丹参素钠含量

【摘要】  目的：建立一种测定心舒颗粒含量的方法。方法：以Shim�pack CLC�ODS(5 μm,4.6 mm×150 mm)为分析柱，甲醇�乙腈�磷酸三乙胺水溶液(4：3：93)为流动相，检测波长281 nm，进样量10 μL。采用HPLC法测定丹参素钠的含量。结果：丹参素钠在0.26 μg-0.62 μg范围内，线性关系：Y=14372866X-137054.2(r=0.9998)，平均回收率为97.32%，RSD=1.66%(n=5)。结论：本法操作简便，结果可靠，重现

Disruption by Fusion PCR

are as follows: 20ng Plasmid template DNA      5ul 5uM Primer1/ m13 forward/ Primer3      5ul 5um Primer2/ m13 reverse/ Primer 4      5ul 10xVent Buffer      5ul 2mM dNTPs      1ul Vent      H2O to 50ul      10X Vent Buffer:      0.2M TRIS pH 8.8      0.1M KCl  

原创：western protocol

Tris(PH=6.8) 10% SDS 40 µl 10% ammonium persulfate 40 µl TEMED 7 µl C. Preparation of gel 1.  Assemble the glass plates and spacers (0.75 mm thick). 2.  Pour the running gel to about 1 cm below the wells of the comb (~3.2 ml). 3.  Seal with 1 ml