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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
详询
- 保质期:
详询
- 英文名:
Corning BioCoat Poly-D-Lysine 96 Well Clear Flat Bottom Microplate, 20/Pack, 80/Case
- 库存:
99
- 供应商:
北京孚博生物
- CAS号:
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- 规格:
80
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文献和实验Isolation, Culture, and Differentiation of Progenitor Cells from the Central Nervous System
in Step 20) Plates, polystyrene, 96-well flat bottom, low cell binding (Corning 3474) (optional; see Step 21) Razor blade, sterile Scissors, sterilized microspring (8.5-cm) (Fine Science Tools 15009-08) Slides, eight-well chamber
dishes (e.g., Corning® CellSTACK®). 6. CO 2 incubator and biosafety cabinet. 7. Filter bottle, 0.5–1 L, 0.2-μm pore size (Corning or equivalent). 2.2. Preparation of Wnt3A CM for Fractionation 1. 20% (v/v) Triton X-100. 2. 1 M Tris-HCl, pH
resuspended in 3XSSC, and printed onto a poly-L-lysine coated slide. Materials, Reagents & Solutions 96 well alkaline lysis miniprep kit (Edge BioSystems, Gaithersburg, MD) LB Broth (Biofluids, Rockville, MD) Superbroth (Biofluids, Rockville
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