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- corning
- 美国
- 356539
- 2026年10月26日
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- 文献和实验
- 技术资料
- 保存条件：
详询
- 保质期：
详询
- 英文名：
Corning® BioCoat™ Poly-D-Lysine 175cm² Flask with Vented Cap
- 库存：
99
- 供应商：
北京孚博生物
- CAS号：
详询
- 规格：
40
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文献和实验Culturing Human Embryonic Stem Cells (hESCs) on MEF-Conditioned Medium
MEF-Conditioned Medium (MEF-CM) 1) Cover the whole surface of each new culture vessel with Attachment Factor (AF) solution and incubate the vessels for 30 minutes at 37°C or for 1 hour at room temperature. For MEF-CM generation, a T-175 flask
4. Add the pre-warmed dissociation reagent such as trypsin or TrypLE™ to the side of the flask; use enough reagent to cover the cell layer (approximately 0.5 mL per 10 cm2 ). Gently rock the container to get complete coverage of the cell layer
Endothelial Cell Systems - Instructions For Use
medium for every 5 cm2 surface area of the flask. Example: 15 ml growth medium for a 75 cm2 flask. d. If not using vented caps, loosen caps of flasks. Place the new culture vessels into a 37・ C humidified incubator with 5% CO2 and equilibrate
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