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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
冷冻-20℃
- 英文名:
1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[cyanur(polyethylene glycol)-2000] ammonium salt
- 库存:
999+
- 供应商:
上海维亦特生物科技有限公司
- CAS号:
1246304-74-4
别名:1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[cyanur(polyethylene glycol)-2000] ammonium salt
吸湿性:Yes
分子式:C135H265Cl2N6O54P (average MW due to polydispersity of PEG)
分子量:2938.467
稳定性:1 Year
存储:冷冻-20°C
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文献和实验Crystallization of Kinesin Family Motor Proteins
temperature11 mg/ml protein 10 mM HEPES pH 7.5, 150 mM NaCl, 1 mM DTT, 1 mM MgCl2, 0.2 mM NaN3, 2 mM ADP combined 1:1 with 22% methyl ether PEG 2000, 100 mM NaCl, 2% ethylene glycol, 50 mM HEPES pH 7.0Gulick et al., 1998Ncd 281-700Sitting drop 4°C20 mg/ml
Materials YPD plates YPD 1 M sorbitol 182 g/l (Sigma S7547) 2 M sorbitol 36.4 g/100 ml SCE (per liter) 1 M sorbitol (182 g) 100 mM citric acid trisodium salt dihydrate (29.4 g)
into acetonitrile/acetic acid solution (5% acetonitrile/0.5% acetic acid). This essential step removes buffer components from the complex peptide mixture (i.e., ammonium bicarbonate, urea, etc.) that are incompatible with ESI-MS/MS. Removal of salt
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