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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
冷冻-20℃
- 英文名:
Cardiolipin Mixture Internal Standard
- 库存:
999+
- 供应商:
上海维亦特生物科技有限公司
- CAS号:
N/A
别名:Cardiolipin Mixture Internal Standard
稳定性:1 year
存储:冷冻-20°C
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文献和实验Kinetic Quantitative PCR vs End-Point Quantitative PCR with Internal Standard
Quantitative PCR can be done either by measuring the amount of PCR products at a given number of cycle (end-point quantitative PCR) (1 –4 ) or by following the amount of products during the PCR at several cycles (kinetic quantitative PCR
and IPG IEF gels, a train of differentially carbamylated proteins producing a uniform series of spots on the 2-D gel provides an internal standard for both evaluating the performance of the isoelectric focusing gel and comparing the 2-D spots positions
making a cocktail of AluI, NlaIII, Sau96I and StyD4I - all frequent cutters - andd adding it to the RT reaction at 1 U ea. enzyme / 20 ul RT reaction. Before RT, the reaction mixture is incubated at 37 deg for 30 min. Then, after RT, the reaction
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