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- 易致生物
- MRI-2000
- 2025年12月03日
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文献和实验system and, indeed, other tissues, is poorly defined. We sought to examine the expression and regulation of the IRF genes in a number of different murine models for immune- or virally induced central nervous system disease. Here, we describe the development of a multiprobe RNase
RNase Protection Assay (Bowtell Lab)
0.5ul 200mM DTT 0.5ul appropriate RNA polymerase Incubate for 90-120min at 41�. 5. Add 0.5ul RNAsin and 0.5ul RNAse free DNAse (5mg/ml). Incubate for 15min at 37�. 6. Add 10ug tRNA and 100ul DDW. Phenol/CHCl3 extract and precipitate with 50ul 5M NH
can then be used as a template for synthesis or radiolabeled anti-sense RNA probes. Standard RPA Procedure In all steps of the protocol, standard precautions should be used to avoid RNase contamination and exposure of personnel to radioactivity. Typically
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