InvivoCrown In vivo CD8+ T cel

InVivoPro Anti-Mouse CD40 in vivo antibody, Clone FGK45InVivoPro Anti-Mouse CD8β (Lyt 3.2) in vivo antibody, Clone 53-5.8Rat IgG2aRat IgG1 κFGK4553-5.8CD40CD8βCD40, Bp50, TNFRSF5Lyt-3.2, Ly-3.2IV0105 or Rat IgG2a Isotype Control Rat IgG1 Isotype ControlPBS, pH 7.2, contains no stabilizers or preservativesPBS, pH 7.2, contains no stabilizers or preservativesMouseMouseRatRat200-500 μg per mouse; or 10-20 mg/kg. This range is based off the most recent publication data using the FGK45 clone in vivo. Each investigator should determine their own optimal working dilution for specific applications.200 μg per mouse; or 10 mg/kg. This range is based off the most recent publication data using the 53-5.8 clone in vivo. Each investigator should determine their own optimal working dilution for specific applications.8489Spitzer, Matthew H. et al. "Systemic Immunity is Required for Effective Cancer Immunotherapy",Cell 168.3 (2017): 487-502.e15. Zhang, Lei et al. "Single-Cell Analyses Inform Mechanisms of Myeloid-Targeted Therapies in Colon Cancer",Cell 181.2 (2020): 442-459.e29. Wu, Renee et al. "Mechanisms of CD40-dependent Cdc1 Licensing Beyond Costimulation",Nature Immunology 208.1_Supplement (2022): 61.18-61.18.Jerby-Arnon, Livnat, et al. "A cancer cell program promotes T cell exclusion and resistance to checkpoint blockade." Cell 175.4 (2018): 984-997. Ringel, Alison E., et al. "Obesity shapes metabolism in the tumor microenvironment to suppress anti-tumor immunity." Cell 183.7 (2020): 1848-1866. Ortiz-Muñoz, Guadalupe et al. "In Situ Tumour Arrays Reveal Early Environmental Control of Cancer Immunity.",NATURE 618.7966 (2023): 827-+.CD40, also known as TNFSF5, is a type I transmembrane protein and member of the TNF receptor family. The binding of CD40L (CD154) on TH cells to CD40 activates antigen presenting cells and induces a variety of downstream effects. CD40 is expressed on B cells, dendritic cells, monocytes, thymic epithelial cells and, at low levels, on T cells. Signaling though CD40 plays an important role in the proliferation and differentiation of B cells and is critical for immunoglobulin (Ig) class switching. The membrane-anchored CD40L is expressed almost exclusively on activated CD4+ T lymphocytes. Failure to express CD40L leads to "immunodeficiency with hyper-IgM", a disease characterized by failure to produce IgG, IgA and IgE. Some of the early intracellular signaling by the CD4-CD40L system includes the association of the CD40 with TRAFs and the activation of various kinases (4). Adaptor protein TNFR2 interacts with this receptor and serves as a mediator of the signal transduction. The interaction of CD4-CD40L is found to be necessary for amyloid-beta-induced microglial activation, and thus is thought to be an early event in Alzheimer disease pathogenesis.CD8b is also known as Lyt-3.2 or Ly-3.2. It is a member of the Ig superfamily expressed as a heterodimer with the CD8A chain on a subset of MHC class I-restricted T cells and most thymocytes.In vivo CD40 activation, in vitro B cell stimulation/activation, Flow cytometryIn vivo CD8+ T cell depletion,Flow cytometry, Western blotprotein A or Gprotein A or GThis antibody is stable for at least 2 months when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at -20°C or -80°C. Avoid repeated freeze thaw cycles.This antibody is stable for at least 2 months when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at -20°C or -80°C. Avoid repeated freeze thaw cycles.2-8°C with blue ice2-8°C with blue iceLot specific, generally ≥ 5.0 mg/mlLot specific, generally ≥ 5.0 mg/ml12 months from the date of receipt if stored as recommended12 months from the date of receipt if stored as recommendedPBS Buffer, PH 7.2, with no carrier protein, or preservatives.PBS Buffer, PH 7.2, with no carrier protein, or preservatives.0.2 μM filtered0.2 μM filtered≤ 1.0 EU/mg, by the LAL method≤ 1.0 EU/mg, by the LAL method0.990.99可替换BioXCell产品货号为BE0223