Soil RNA Midi Kit R6826

E.Z.N.A.® Total RNA Midi Kit Protocol for Eukaryotic Cells and Tissues

-milling or rotor-stator homogenizer, samples are then applied to the HiBind® Midi spin columns to which total RNA binds, after few quick washing step, cellular debris and other contaminants are effectively washed away. High quality RNA is finally eluted

RNEasy Midi

  Materials Qiagen RNEasy Midi Kit Dounce homogenizers (for tissues) Syringes and needles (for cultured cells) 70% EtOH Sterile 15 mL conical tubes   Procedure 1) Before using the reagents

Using Soil DNA Kit (D5625) to Clean Up DNA containing inhibitors

3. Centrifuge at full speed (>13,000 x g) for 2 minutes. 4. Transfer cleared supernatant to a new 1.5 ml tube. Note: If the supernatant still shows dark color from soil at this point, perform the HTR extraction again by repeating step 1-4.