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文献和实验The E.Z.N.A.® Mag-Bind Dye terminator Removal Procedure
1. Determine the volume of the crude sequencing product and transfer the sample into 96-well microplate. Add 10 ul molecular grade water to each sample. 2. Add 2 volume of Mag-Bind® Particle/ethanol mixture and mix thoroughly by pipetting
Sequenase[TM] catalyzed sequencing with dye-labeled terminators
fragments are precipitated and dried. To aid in the removal of unincorporated dye-terminators, the DNA pellet is rinsed twice with ethanol. The dried sequencing reactions could be stored up to several days at -20degC. Double-stranded dye-terminator
Sequencing off Bacterial Genomic DNA with ABI Big Dye Terminators
The following protocol and cycle conditions courtesy of Cheryl Heiner-ABI--------------------------------------------------------------------------------Protocol:Big Dye Terminator Mix* 16μlThermoFidelase** 1μlPrimer (18-24mer)15-30pmolesGenomic DNA
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