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文献和实验DNA WALKING REFERRENCEKi-Bum Park and Suk-Heung Oh(2007)Cloning, sequencing and expression of a novel glutamate decarboxylase gene from a newly isolated lactic acid bacterium, Lactobacillus brevis OPK-3. Bioresource Technology 98(2): 312-319.Young
. 1B). Infection efficiency and expression of individual library members were tracked with the green fluorescent protein (GFP) marker. Cells overexpressing a target gene product on the cell surface were selected by flow cytometry. The DNA encoding
Preparation of Poly A+ RNA and Northern Analysis
Protocols; leave out Percoll step) first, then extract mRNA. To extract from cultured cells (ie. HT1080), begin with one T75 flask (approx. 2 x 106 cells). 2) Have Pharmacia kit reagents warmed to RT for 30 min. Check if Extraction buffer is in solution
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