- ￥1176 - 9828
- abinScience
- France
- HX879013
- 2026年08月30日
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- 文献和实验
- 技术资料
- 保存条件：
-20℃
- 保质期：
12 months
- 英文名：
Anti-Human Di-acetylated histone H4/H4K5acK8ac Antibody (1A9D7)
- 供应商：
武汉益普生物科技有限公司
- 规格：
50μg/100μg/1mg
| 规格： | 50μg | 产品价格： | ￥1176.0 |
|---|---|---|---|
| 规格： | 100μg | 产品价格： | ￥1956.0 |
| 规格： | 1mg | 产品价格： | ￥9828.0 |
| 产品名称 | Anti-Human Di-acetylated histone H4/H4K5acK8ac Antibody (1A9D7) |
| 货号 | HX879013 |
| 描述 | Anti-Human Di-acetylated histone H4/H4K5acK8ac Antibody (1A9D7) [1A9D7] ( HX879013 ) is a mouse monoclonal antibody detecting HIST1H4D in ELISA, WB . Suitable for Human . |
| 反应种属 | Human |
| 应用实验 | ELISA, WB |
| 宿主/表达体系 | Mouse |
| 靶点 | HIST1H4D, HIST2H4A, H4FE, H4FO, H4/D, H4/A, HIST1H4E, HIST1H4F, H4FG, HIST1H4L, H4FD, H4/H, HIST1H4B, HIST1H4I, H4FN, HIST1H4C, H4FI, H4FC, H4/C, H4/E, Histone H4, H4/G, H4/O, H4FB, H4FK, H4/M, H4/B, H4C1, HIST2H4, H4F2, HIST2H4B, HIST1H4A, HIST1H4J, H4/N, H4FH, H4/I, H4/J, H4/K, H4FJ, HIST1H4H, HIST1H4K, HIST4H4, H4FM, H4FA |
| 基因号 | P62805 |
| 状态 | Liquid |
| 纯度 | >95% as determined by SDS-PAGE. |
| 储存温度 | -20℃ |
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文献和实验Identifying Acetylated Proteins in Mitosis
to search for acetylated proteins in mitosis (2). First, we synchronized cells in mitosis and used a polyclonal anti-acetyl-Lysine antiserum to immunoprecipitate acetylated proteins, followed by their identification by LC-ESI-MS/MS. We then confirmed
in 100µl of elution buffer at 65°C for 5 minutes;7.The eluted complexes can then be electrophoresed in denaturing PAGE and subjected to western blot analysis with an anti-Flag antibody for detection of the particular flag-tagged protein of choice (Figure
Chromatin Immunoprecipitation Protocol for Histone Modification
. This has been very successful when using antibodies that detect site specific histone lysine methylation (Peters et al . 2003, Martens et al . 2005). However, for each different antibody the optimal concentration should be tested and calibrated. The stringency of washing
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