- ￥1176 - 9828
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- HA916013
- 2026年08月30日
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- 文献和实验
- 技术资料
- 保存条件：
-20℃
- 保质期：
12 months
- 英文名：
Anti-BBS3/Arl6 Antibody (N302/10)
- 供应商：
武汉益普生物科技有限公司
- 规格：
50μg/100μg/1mg
| 规格： | 50μg | 产品价格： | ￥1176.0 |
|---|---|---|---|
| 规格： | 100μg | 产品价格： | ￥1956.0 |
| 规格： | 1mg | 产品价格： | ￥9828.0 |
| 产品名称 | Anti-BBS3/Arl6 Antibody (N302/10) |
| 货号 | HA916013 |
| 描述 | Anti-BBS3/Arl6 Antibody (N302/10) [N302/10] ( HA916013 ) is a mouse monoclonal antibody detecting Bardet-Biedl syndrome 3 protein in IHC, WB . |
| 应用实验 | IHC, WB |
| 宿主/表达体系 | Mouse |
| 靶点 | Bardet-Biedl syndrome 3 protein, BBS3, ADP-ribosylation factor-like protein 6, ARL6 |
| 状态 | Liquid |
| 纯度 | >95% as determined by SDS-PAGE. |
| 储存温度 | -20℃ |
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文献和实验Monoclonal Antibody Production Protocol
the rinse twice. Add 100 ul of blocking solution to every well, leave 1 hr at room Temp or O.N at 4°C. PRIMARY ANTIBODY Add the antibody to be tested: Sup of cells = 25 ul, mix well by pipetting up and down (10 times).serum, ascites = 1:100 and a series
monoclonal antibody production fusion
, add ddH2 O up to 1 L Gluta x50, 1 L, pH 6.0 = glutamine, 10.8 g (1.48 mM) + HCl, 0.5N, 100 ml -> heat to 37ѓC, add ddH2 O up to 1 L aminopterine x100, 0.22-0.45 µm-filtered, store at -20ѓC = aminopterine, 4.4 mg (3.8µM)+ ddH2 O
Protocol for anti-HA antibody Western Blotting
of interest. 2) Transfer proteins in acrylamide gel to nylon membrane. 3) Block overnight @4℃ in PBS (or TBS, it doesn't matter) with 5% non-fat dry milk. I dilute 2.5 mg in 50 ml. 4) Wash off excess milk sol'n with PBS x3 .(important if you are re-using your antibody
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