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- 详细信息
- 文献和实验
- 技术资料
- 亚型:
IgM
- 形态:
Liquid
- 保存条件:
-20°C
- 克隆性:
单克隆
- 适应物种:
Mouse, Rat, Porcine
- 保质期:
12 months
- 级别:
一抗
- 供应商:
武汉益普生物科技有限公司
- 宿主:
Mouse
- 应用范围:
ELISA, FCM, FuncS, IHC-P, WB
- 浓度:
1.04 mg/ml
- 抗体英文名:
Anti-α-Gal Epitope (Galα1-3Galβ1-4GlcNAc-R) Antibody (M86)
- 规格:
50μg/100μg/1mg
| 规格: | 50μg | 产品价格: | ¥1338.0 |
|---|---|---|---|
| 规格: | 100μg | 产品价格: | ¥2288.0 |
| 规格: | 1mg | 产品价格: | ¥11148.0 |

| Product name | Anti-α-Gal Epitope (Galα1-3Galβ1-4GlcNAc-R) Antibody (M86) |
| Catalog No. | YP493013 |
| Target | α-Galactose 1,3, α-Gal Epitope (Galα1-3Galβ1-4GlcNAc-R),alpha-gal, alpha gal, Gal |
| Clonality | Monoclonal |
| Host species | Mouse |
| Species reactivity | Mouse, Rat, Porcine |
| Applications | ELISA, FCM, FuncS, IHC-P, WB |
| Isotype | IgM |
| Form | Liquid |
| Storage buffer | 0.01M PBS, pH 7.4. |
| Concentration | 1.04 mg/ml |
| Purity | >95% as determined by SDS-PAGE. |
| Purification | Protein L purified from cell culture supernatant. |
| Endotoxin level | Please contact with the lab for this information. |
| Stability and Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term. |
| Note | Do not use non-fat milk for blocking! Many blocking agents from non-human mammal sources will cross-react with the antibody. |

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文献和实验Immunofluorescence: Rabbit Anti-Murine RELMα
. Following fixation, the tissue was embedded in paraffin and cut into 5 μM sections. 1. Deparaffinize and rehydrate the tissue section. 2. Perform heat-induced antigen retrieval by boiling the tissue section in 10mM pH 6.0 citrate buffer
识别糖链是NeuAcα2-3Galβ1-4GlcNAc β1-R、NeuAcα2-3Gal β1-4Glc β1-R。非还原末端NeuAcα2-3Galβ1-4GlcNAcβ1-R的糖链结构同时存在糖脂和糖蛋白。另外,NeuAc α 2-3Galβ1-4Glcβ1-R的糖链结构是糖脂特殊结构。综上所述,我们通过免疫印迹法分析HYB4抗体对糖蛋白的反应性,结果显示,HYB4抗体对糖蛋白、糖脂两方都有的非还原末端α 2-3结合型唾液酸糖链有反应,特别是存在于糖蛋白上的N-结合型或者O-结合型糖
Epitope Mapping by Chemical Fragmentation
The use of antigen fragments generated by specific chemical cleavage is a relatively simple “library” approach for epitope mapping in which overlapping fragments are screened with antibody on Western blots. It is widely applicable insofar
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