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- BioLegend
- 143909
- 美国
- 2025年07月13日
- 免疫荧光，流式
- 大鼠
- 小鼠
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- 技术资料
- 抗体名：
PE/Cyanine7 anti-mouse CD63
- 抗体英文名：
PE/Cyanine7 anti-mouse CD63
- 浓度：
0.2 mg/ml
- 应用范围：
免疫荧光，流式
- 宿主：
大鼠
- 供应商：
上海善然生物
- 库存：
现货
- 抗原来源：
大鼠
- 保质期：
2-3年左右
- 适应物种：
小鼠
- 标记物：
PE/Cyanine7
- 克隆性：
单克隆
- 保存条件：
2-8度
- 形态：
液体
- 规格：
25ug
PE/Cyanine7 anti-mouse CD63 Antibody
NVG-2
Catalog# / Size 143909 / 25 µg
143910 / 100 µg
Clone
Regulatory Status RUO
Other Names LIMP, LAMP-3, gp55, melanoma-associated antigen, ME491
Isotype Rat IgG2a, κ
Description CD63, also known as LIMP, LAMP-3, gp55, and melanoma-associated antigen (ME491), is a
member of the tetraspanin superfamily (TM4SF) that constitutes a main component of the
lysosomal membrane. It is expressed on activated platelets, monocyte/macrophages,
endothelium, fibroblasts, osteoblasts, and smooth muscle cells. CD63 may be involved in platelet
activation and is thought to function as a transmembrane adaptor protein. CD63 has been shown
to associate with CD9, CD81, VLA-3, and VLA-6. In mice, there are two CD63 gene loci, of which
only one is functional. CD63 deficient mice are viable, and there is no alteration in the population
of immune cells. A recent report shows that CD63-deficient mice exhibit a significant reduction in
both leukocyte rolling and recruitment in a peritonitis model.
Product Details
Verified Reactivity Mouse
Antibody Type Monoclonal
Host Species Rat
Immunogen Intestinal lamina propria light-density cells (enriched with eosinophils)
Formulation Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation The antibody was purified by affinity chromatography and conjugated with PE/Cyanine7 under optimal
conditions.
Concentration 0.2 mg/mL
Storage & Handling The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged
exposure to light. Do not freeze
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Characterization of Whole Body Cholesterol Fluxes in the Mouse
in this pathway. This article describes in detail a variety of experimental approaches to measure cholesterol fluxes in the hepatobiliary system as well as in the intestinal pathway. Curr. Protoc. Mouse Biol. 1:413?427 © 2011 by John Wiley & Sons
Immunofluorescent Staining of Mouse and Rat Leukocytes
-up, please see description in "Procedure for Setting Compensation for Multi-Color Flow Cytometric Analysis". For reducing FcgII/IIIR-mediated antibody binding (or binding of SAv-PE or SAv-Cy-Chrome) which could contribute to background, the use of anti-mouse CD32/CD
Phycoerythrin conjugation protocol
and Centricon concentrators. Add 0.5% sodium azide. Store at 4 °C protected from light. Do not freeze. Figure 1. Analysis of column eluate fractions from PE conjugation of monoclonal antibody IB4 (murine IgG2a, anti-human CD
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