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- 禾午生物
- P71058
- 国产
- 2025年07月14日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
99
- 英文名：
pT3-EF1a-Kras(mouse)-G12D-3×FLAG-IRES-luc
- 保质期：
3个月~12个月
- 供应商：
上海禾午生物有限公司
- 保存条件：
负20℃＆负80℃
- 规格：
干粉＆甘油菌
| 名称 | pT3-EF1a-Kras(mouse)-G12D-3×FLAG-IRES-luc |
| 别称 | NM_021284.7;AI929937/c-K-ras/c-Ki-ras/K-ras/K-Ras/K-Ras 2/Ki-ras/Kras-2/Kras2/p21B/ras |
| 基因长 | 564bp |
| 质粒宿主 | 哺乳细胞 |
| 质粒用途 | 蛋白表达 |
| 片段类型 | ORF |
| 片段物种 | 小鼠 |
| 原核抗性 | Amp |
| 荧光标记 | Fluc |
| 启动子 | EF1a |
| 复制子 | pUC |
| 感受态 | DH5a |
| 温度 | 37度 |
| 背景载体 | pT3-EF1A-MYC-IRES-luc |
| 正向引物 | PEF-F |
| pCMV-ACHE(human)-Neo |
| pCMV-TRIM25(human)-3×Myc-Neo |
| PiggyBac-CMV-MEIS3(human)-CopGFP-Puro |
| pCMV-PARK7(human)-Myc |
| pCMV-ATL2(human)-3×FLAG-Neo |
| pCMV-ARHGAP10(human)-FLAG-Neo |
| pCMV-ZBED1(human)-3×HA-Neo |
| pCMV-IREB2(human)-3×HA-Neo |
| pCMV-App(mouse)-Neo |
| pCMV-VPS4A(human)-EGFP-Neo |
| pCD73(human)-Fluc |
| pPGK-Fluc-Tk1(mouse)-3UTR-SV40-hRluc-Neo |
| pGL3-U6-sgRNA-PGK-mCherry |
| pCMV-WWP2(human)-3×HA-Neo |
| pCMV-Trem2(rat)-3×FLAG-Neo |
| pCMV-PIP5K1A(human)-3×FLAG-Neo |
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文献和实验Blastocyst embryo transfer into pseudopregnant recipient female mouse（图）
and exhibit bad planes of anesthesia when heavy. This is also a very inexpensive mouse to use. As an alternate, another strain I have used with considerable success is B6D2F1. These mice are hardy and display hybrid vigor. The Equipment For surgery
PROTOCOL General anesthesia inject i.p. 250µl/mouse of the ANESTHETIC mixture for verify the anesthetic effect check plantar reflexes (animal must be unresponsive to squeezing hind feet) Preparation
Vasectomized males are needed to engender pseudopregnancy in mature female mice. Such pseudopregnant females act as hormonally and physiologically competent recipients for microinjected fertilized one-cell eggs (see Chapter 20 ). Vasectomized
技术资料