- ￥4104
- BioLegend
- 108142
- 美国
- 2025年07月13日
- 免疫荧光，流式
- 大鼠
- 小鼠
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- 详细信息
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- 技术资料
- 抗体名：
Alexa Fluor® 700 anti-mouse Ly-6A/E (Sca-1)
- 抗体英文名：
Alexa Fluor® 700 anti-mouse Ly-6A/E (Sca-1)
- 浓度：
0.2 mg/ml
- 应用范围：
免疫荧光，流式
- 宿主：
大鼠
- 供应商：
上海善然生物
- 库存：
现货
- 抗原来源：
大鼠
- 保质期：
2-3年左右
- 适应物种：
小鼠
- 标记物：
Alexa Fluor® 700
- 克隆性：
单克隆
- 保存条件：
2-8度
- 形态：
液体
- 规格：
100 μg
Alexa Fluor® 700 anti-mouse Ly-6A/E (Sca-1) Antibody
D7
Catalog# / Size 108141 / 25 µg
108142 / 100 µg
Clone
Regulatory Status RUO
Other Names Sca-1
Isotype Rat IgG2a, κ
Description Ly-6A/E, also known as Sca-1, is an 18 kD member of the Ly-6 multigene family. Ly6A/E is a
glycosylphosphatidylinositol (GPI)-linked protein expressed on hematopoietic stem cells. In mice
expressing the Ly-6.2 haplotype (e.g., AKR, C57BL, C57BR, DBA/2, SJL, SWR, and 129), Ly6A/E is also expressed on peripheral B lymphocytes and thymic and peripheral T lymphocytes.
Strains expressing the Ly-6.1 haplotype (e.g., BALB/c, CBA, C3H/He, DBA/1, and NZB) have low
Ly-6A/E expression on resting peripheral lymphocytes. The expression of Ly-6A/E on lymphocytes
is upregulated upon activation from both Ly6.1 and Ly6.2 haplotype mice. Ly-6A/E is thought to be
involved in the regulation of both T and B cell responses.
Product Details
Verified Reactivity Mouse
Antibody Type Monoclonal
Host Species Rat
Immunogen IL-2-dependent mouse T-cell line (CTL-L)
Formulation Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 700 under
optimal conditions.
Concentration 0.5 mg/ml
Storage & Handling The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged
exposure to light. Do not freeze.
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D Granulocytic : Gr-1 (Ly-6G) IgG2b, 01211A D8 IgG2b, 09821D Eryth: Ter-119 IgG2b, 09081A Sca-1 PE-IgG2a (E31-161.7) 01835B PE-IgG2a Isotype control, 11025
细胞仪检测。或者继续 E1 步 DNA 染色。 E. DNA 染色（可选） 1. 将细胞重悬在 0.5 ml DNA 染色液中（例如 DRAQ5® #4084）。 2. 室温至少孵育 5 分钟。 3. 流式细胞仪进行分析。 ☀ 推荐的二抗： 抗兔： Anti-Rabbit IgG（H+L）， F（ab'）2 Fragment（Alexa Fluor® 488 Conjugate）#4412 Anti-Rabbit IgG（H+L）， F（ab
marker-neurofilament (NF) and synaptophysin antibodies; postsynaptic acetylcholine receptors (AChRs) were labeled with Alexa Fluor 594-conjugated α-bungarotoxin (α-BTX). Immunofluorescence-stained diaphragms were examined under a confocal microscope.
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