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- 详细信息
- 文献和实验
- 技术资料
- 抗体名:
APC anti-mouse CD197 (CCR7)
- 抗体英文名:
APC anti-mouse CD197 (CCR7)
- 浓度:
0.2 mg/ml
- 应用范围:
免疫荧光,流式
- 宿主:
大鼠
- 供应商:
上海善然生物
- 库存:
现货
- 抗原来源:
大鼠
- 保质期:
2-3年左右
- 适应物种:
小鼠
- 标记物:
APC
- 克隆性:
单克隆
- 保存条件:
2-8度
- 形态:
液体
- 规格:
25ug
APC anti-mouse CD197 (CCR7) Antibody
4B12
Catalog# / Size 120107 / 25 µg
120108 / 100 µg
Clone
Regulatory Status RUO
Other Names C-C chemokine receptor type 7 (CCR7), MIP-3 beta receptor, EBV-induced G protein coupled
receptor 1, EBI-1, CD197
Isotype Rat IgG2a, κ
Description CD197 is also known as C-C chemokine receptor 7 (CCR7) or EBI-1. CCR7 is a G-coupled
rhodopsin-like member of the GPCR superfamily with a predicted molecular weight of 43 kD
that is expressed on hematopoietic stem cells, most naive T cells, some memory T cells, B
subset, and mature dendritic cells. CCR7 is a receptor for the chemokines CCL19 (MIP3 beta)
and SLC (6CKine, Exodus-2, TCA-4, CCL21) that plays a role in thymocytes development, T
cell adhesion at intestinal sites, the homeostatic recirculation of memory T cells, and
chemotaxis.
Product Details
Verified Reactivity Mouse
Antibody Type Monoclonal
Host Species Rat
Immunogen Mouse CCR7 transfected RBL-2H3 cells
Formulation Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation The antibody was purified by affinity chromatography, and conjugated with APC under optimal
conditions.
Concentration 0.2 mg/ml
Storage & Handling The antibody solution should be stored undiluted between 2°C and 8°C, and protected from
prolonged exposure to light. Do not freeze.
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流式课堂 | 易被忽略的 CD4、CD8 共表达细胞——胸腺淋巴细胞
做过流式实验的同学,对 CD4、CD8 这两个指标都不会陌生。检测 CD4+ T 细胞和 CD8+ T 细胞分型,是流式中最常见的实验之一。 通常,我们会先检测 CD3,来圈总T淋巴细胞,再分别用 CD4 抗体和 CD8 抗体,来区分 CD4+ T 淋巴细胞(辅助或调节性T细胞)和 CD8+ T 淋巴细胞(细胞毒性 T 细胞)。 新生小鼠脾脏细胞 上图为新生小鼠脾脏细胞进行的 CD3-PE、CD4-FITC、CD8-APC 三色流式实验结果。从图中可以看出,在新生小鼠脾脏中,绝大
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