- ￥1000 - 5000
- Invent
- 美国
- WA-008
- 2025年07月15日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
4℃
- 保质期：
见包装
- 英文名：
Invent Minute WB Blocking Solution
- 库存：
999
- 供应商：
北京泽平
- 规格：
250ml
货号：WA-008
中文名称：Minute WB封闭试剂
英文名称：Minute WB Blocking Solution
包装规格：250ml
品牌：英文特 Invent Biotechnologies
产地：美国
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文献和实验Immunocytochemistry Protocol Using Anti-GFP Antibodies(使用抗GFP抗体的免疫细胞化学实验方案)
the cells twice in PBS for 1 minute each with gentle agitation. Remove the PBS. 6. Add Blocking solution (5% Normal Goat Serum in PBS, pH 7.4) to the cells. Incubate for 1 hour at room temperature with gentle agitation. Remove the solution
Wholemount staining of embryos
) at room temperature for 15 to 30''. Use nutator (Clay Adams, Fisher cat#14-062) Remove blocking solution and add primary antibody (500 ul), incubated overnight at 4 degC if sensitivity is not a problem. Antibody is added either as a cell culture
-T►Add primary antibody diluted into blocking solution. Incubate at room►temp for 1 hour. (Can be left at 4℃ O/N here)►Wash briefly 2X then 3X10 min each with TBS-T►Add secondary HRP conjugated antibody diluted into blocking solution. Incubate at room
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