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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
4℃
- 保质期:
见包装
- 英文名:
Invent Minute Detergent-Free Plant Protein Extraction Kit
- 库存:
999
- 供应商:
北京泽平
- 规格:
4次,50次
货号:SN-010
中文名称:Minute无表面活性剂的植物组织总蛋白提取试剂盒
英文名称:Minute Detergent-Free Plant Protein Extraction Kit
包装规格:4次,50次
品牌:英文特 Invent Biotechnologies
产地:美国

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文献和实验Protein extraction from whole tissues for IEF
such as plants (see Hurkman and Tanaka, 1986, Plant Physiology 81:802-806). Grind 1 g of fresh tissue to a powder with liquid nitrogen in a mortar and pestle. Add 2.5 ml of Tissue Extraction Medium and continue grinding for an additional minute
-Orbitrap/MS proteomics workflow. The compatibility of this protocol with high concentrations of detergents significantly increases the extraction yield and the abundance of membrane proteins while gel fractionation increases the number of protein
Protocols for protein extraction from whole tissues for isoelectric focusing
)that tolerate detergents and reducing agents. Phenol extraction followed by methanolic ammonium acetate precipitation – an effective protocol for sample preparation from protein-poor,recalcitrant tissues such as plants (see Hurkman and Tanaka,1986,Plant
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