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- 文献和实验
- 技术资料
- 保存条件:
Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.
- 保质期:
12个月
- 英文名:
Recombinant human TNFSF15 Protein with N-terminal 10×His tag
- 库存:
In Stock
- 供应商:
缔码生物(武汉)科技有限公司
- 规格:
10μg
| 产品类型 | ECD Proteins |
|---|---|
| 靶点 | TNFSF15 |
| Uniprot ID | O95150 |
| 背景 | The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This protein is abundantly expressed in endothelial cells, but is not expressed in either B or T cells. The expression of this protein is inducible by TNF and IL-1 alpha. This cytokine is a ligand for receptor TNFRSF25 and decoy receptor TNFRSF21/DR6. It can activate NF-kappaB and MAP kinases, and acts as an autocrine factor to induce apoptosis in endothelial cells. This cytokine is also found to inhibit endothelial cell proliferation, and thus may function as an angiogenesis inhibitor. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2011] |
| 货期 | In Stock |
| 产品英文名称 | Recombinant human TNFSF15 Protein with N-terminal 10×His tag |
| 分子结构 | 10×His tag TNFSF15(Leu72-Leu251) |
| 分子量 | The protein has a predicted molecular mass of 21.8 kDa after removal of the signal peptide. |
| 纯度 | The purity of the protein is greater than 85% as determined by SDS-PAGE and Coomassie blue staining. |
| 无菌性 | Products are supplied non-sterile. For cell culture applications, dilute in appropriate medium and sterile-filter (0.22 µm) prior to use. |
| 储存与运输 | Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
| 别名 | TL1; TL1A; VEGI; TNLG1B; VEGI192A |
| 标签 | N-10×His tag |
| 有无偶联 | Unconjugated |
| 表达宿主 | HEK293 |
| 组分 | Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution. |
| 应用范围 | Research use only |

Figure 1. Human TNFSF15 Protein, N-His Tag on SDS-PAGE under reducing condition.

Figure 2. ELISA plate pre-coated by 2 μg/mL (100 μL/well) Human TNFSF15 Protein, N-His Tag (PME101723) can bind Anti-TNFSF15(PF-06480605 biosimilar) mAb (BME100222) in a linear range of 1.6–40 ng/mL.
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文献和实验表达 • N端带有6×His标签,在Ni-NTA上进行一步亲和纯化, 利用TAGZyme™可去除6×His标签 • 可表达无标签蛋白,利用Nde I酶切,再连接去除His标签 • C端内置琥珀终止密码子,可利用EasyXpress Site-Specific Biotin Kit进行生物素标记 • 卡那霉素抗性用于筛选 图1 QIAgenes expression vector – E.coli QIAgenes表达载体有效提高蛋白表达量 说了这么多,还是通过实验数据来具体
一,酵母表达系统毕赤酵母表达系统(invitrogen公司):分泌型质粒pPIC9K,宿主菌毕赤酵母GS115分泌型质粒pPICZalpha (甲醇诱导)宿主菌毕赤酵母x33分泌型质粒pHIL-S1二,原核表达质粒表达宿主菌为BL21(DE3)系列带His标签(N端):PET5-(a)氨苄抗性带His标签(N端):PET28-(a)卡那抗性带His标签(C端):PET24-(b)卡那抗性带His标签(C端):PET21-(a)氨苄抗性带His标签(N端)+TrxA(硫氧还蛋白):PET-32
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