重组人瘦素蛋白

重组人瘦素蛋白 | Human Leptin Protein (PRP1038)

核心参数

产品类型：细胞因子（无标签）
种属来源：人源表达
纯度验证：> 98% (SDS-PAGE分析)
分子量测定：16 kDa（146个氨基酸组成）
表达系统：大肠杆菌高效表达体系
结构特征：Val22-Cys167蛋白片段
内毒素：<1.0 EU/μg (LAL法检测)
制剂形态：冻干粉（20 mM Tris，150 mM NaCl，pH7.4）
应用方向：细胞代谢调控研究、肥胖机制分析

产品描述

本重组蛋白通过大肠杆菌表达系统制备，精确复现人源Leptin的天然构象，适用于体外细胞培养体系中的受体结合实验、信号通路研究及代谢调控机制探索。

技术优势

• 高批间一致性：严格质控确保蛋白稳定性
• 即用型制剂：预优化缓冲体系可直接用于细胞实验
• 无标签干扰：保留天然蛋白活性结构

使用指南

• 复溶前3000rpm离心2分钟
• 推荐复溶浓度≥100μg/mL
• 工作液建议现配现用

存储方案

• 未开封：-20℃以下干燥保存
• 复溶后：4℃稳定7天，-20℃长期保存
• 冻存建议：添加0.2% HSA/BSA分装保存

包装规格

规格	价格	货期
100 µg	¥588	现货
1 mg	¥1588	定制生产

运输保障

全程蓝冰冷链运输，确保生物活性

重要声明

本产品仅限于科研用途，不适用于临床诊断。使用前请充分阅读相关文献资料，确认符合实验伦理要求。

生物学背景

瘦素（UniProt ID：P41159）作为脂肪细胞分泌的关键代谢调节因子，通过血脑屏障作用于下丘脑受体，精准调控能量代谢平衡。其浓度变化直接反映机体能量储备状态，是代谢综合征研究的核心靶点。

参考信息

Gene ID：3952 | NCBI Accession：NP_000221

注意：以上信息可能随着产品优化升级等因素产生变动，具体信息请咨询亚科因公司。

Recombinant Human Leptin Protein | Human Leptin Protein (PRP1038)

Key Parameters

Product Type: Cytokine (Tag-Free)
Species Source: Human Expression
Purity Verification: > 98% (SDS-PAGE Analysis)
Molecular Weight Determination: 16 kDa (146 Amino Acids)
Expression System: High-Efficiency E. coli Expression System
Structural Feature: Val22-Cys167 Protein Fragment
Endotoxin: <1.0 EU/μg (LAL Method)
Formulation: Lyophilized Powder (20 mM Tris, 150 mM NaCl, pH7.4)
Application: Cell Metabolism Regulation Research, Obesity Mechanism Analysis

Product Description

This recombinant protein is prepared using an E. coli expression system, accurately reproducing the natural conformation of human Leptin. It is suitable for receptor binding experiments, signaling pathway research, and metabolic regulation mechanism exploration in in vitro cell culture systems.

Technical Advantages

• High Batch-to-Batch Consistency: Strict quality control ensures protein stability
• Ready-to-Use Formulation: Pre-optimized buffer system for direct use in cell experiments
• Tag-Free Interference: Preserves the natural protein active structure

Usage Guide

• Centrifuge at 3000 rpm for 2 minutes before reconstitution
• Recommended reconstitution concentration ≥100 μg/mL
• Working solution should be prepared fresh

Storage Solution

• Unopened: Store dry at -20°C or below
• After Reconstitution: Stable at 4°C for 7 days, long-term storage at -20°C
• Freezing Recommendation: Add 0.2% HSA/BSA for aliquot storage

Packaging Specifications

Specification	Price	Lead Time
100 µg	¥588	In Stock
1 mg	¥1588	Custom Production

Transportation Assurance

Blue ice cold chain transportation throughout to ensure biological activity

Important Statement

This product is for research use only and is not suitable for clinical diagnosis. Please read relevant literature thoroughly before use to ensure compliance with experimental ethical requirements.

Biological Background

Leptin (UniProt ID: P41159), as a key metabolic regulator secreted by adipocytes, acts on hypothalamic receptors through the blood-brain barrier to precisely regulate energy metabolism balance. Its concentration changes directly reflect the body's energy reserve status, making it a core target in metabolic syndrome research.

Reference Information

Gene ID: 3952 | NCBI Accession: NP_000221

Note: The above information may change due to product optimization and upgrades. For specific information, please consult Abbkine Company.