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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
详询
- 保质期:
详询
- 英文名:
HIS Lite™ Cy3 Tris NTA-Ni Complex
- 库存:
99
- 供应商:
北京孚博生物
- CAS号:
详询
- 规格:
100ug
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文献和实验Co-Expression of human DNA primase p49-p58 subunits
) •3mM BME (to prevent disulfide formation,Ni beads are compatible w/low concs.of BME,but NOT DTT.) Wash buffer: •1% Triton X-100 •50mM Tris,pH 8.0 •300mM NaCl (Qiagen recommends at least 300mM NaCl to prevent ionic interactions w/Ni-NTA beads.NaCl
with the better the chances of a successful pulldown. A total of 4.0×106 293 HEK cells (~70% confluent) are plated and 24 hours later transfected with 15µg of a RNA Polymerase II expressing flag-tagged construct plasmid. We generally use Lipofectamine 2000™
Studies of the Ubiquitin Proteasome System
3 Basic Protocol 14: Purification of APP‐BP1/Uba3 Complex (E1 for NEDD8) Expressed Using the Baculovirus Expression System Materials NEDD8 E1 on Ni‐NTA agarose beads ( protocol 21 ) Wash buffer
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