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- 保存条件:
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- 英文名:
HIS Lite™ iFluor® 647 Tris NTA-Ni Complex
- 库存:
99
- 供应商:
北京孚博生物
- CAS号:
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- 规格:
100ug
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文献和实验of tissue/ cell harvest. Dynabeads® mRNA DIRECT™ Kit protocols can be scaled up or down to suit specific sample source and quantity. Please see section "Sample Guidelines and Scaling" before preparing the sample, and for recommended bead and buffer volumes
Dynabeads® mRNA DIRECT Micro Kit - mRNA isolation for RT-PCR amplification
实验原理 Use the Dynabeads® mRNA DIRECT™ Micro Kit for simple and rapid isolation of mRNA from small cell and tissue samples, for direct use in reverse transcription PCR. The direct mRNA isolation is completed in only 15
-Buffer® STRONG and incubate (2 h) 100 µl per well on the plate 8. Wash three times with 300 µl 1-fold Washing Buffer TRIS per well 9. Incubate (1 h) with detector conjugate stored in HRP-Protector™ 10. Detect with substrate after further washing
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