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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
详询
- 保质期：
详询
- 英文名：
ReadiUse™ Tyramide (TSA)/Styramide (PSA) Optimized Reaction Buffer
- 库存：
99
- 供应商：
北京孚博生物
- CAS号：
详询
- 规格：
1000Reactions
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文献和实验, hybridization, antibody detection, POD–TSA reaction, and mounting of embryos for imaging. To achieve high signal intensities, we optimized key steps of the method. This includes improvement of embryo permeability by hydrogen peroxide treatment and efficacy
Sequential RNA and DNA fluorescence in situ hybridization
, can cause significant loss of RNA FISH signals. To overcome this problem, we have employed tyramide signal amplification (TSA) to detect RNA FISH signals in a combined DNA/RNA FISH protocol in which RNA is first detected followed by DNA detection
: Cy3 tyramide conjugates (1/50 dilution of stock solution in amplification buffer; Perkin Elmer Life Sciences, Boston, MA, USA; Catalog No. SAT704A) or Alexa Fluor 488 tyramide conjugate (1/50 dilution of stock solution in amplification buffer
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