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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
详询
- 保质期：
详询
- 英文名：
Cal-630™-Dextran Conjugate *MW 10,000*
- 库存：
99
- 供应商：
北京孚博生物
- CAS号：
详询
- 规格：
1mg
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文献和实验Phycoerythrin conjugation protocol
concentrator (Centricon 30 or 100 are fine) Apply to top of separation column. Elute with PBS. Pump column at approximately 0.3 ml/min. Order of elution is conjugate first (MW ~400,000) then free PE (MW ~240,000) then free Ab (MW ~150,000
IMMUNOHISTOCHEMISTRY ON WHOLE MOUNT EMBR
, although my current view is that this is not necessary. I use Tago G anti- Ms, Rb and Rt. I use HRP-avidin and AP-avidin from BRL @ 1:2000 and 1:1000, respectively. Add avidin-enzyme conjugate in PBT, rotate 30''. Wash 3X in PBT with a final wash in AP
IMMUNOHISTOCHEMISTRY ON WHOLE MOUNT EMBRYOS
conjugate in PBT, rotate 30'. Wash 3X in PBT with a final wash in AP buffer if using AP-conjugate.. The AP staining conditions are as described in the BRL handout that comes with the conjugate. Although the AP stain is more sensitive than HRP
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