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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
详询
- 保质期：
详询
- 英文名：
Amplite® Colorimetric Glycerol 3-Phosphate (G3P) Assay Kit
- 库存：
99
- 供应商：
北京孚博生物
- CAS号：
详询
- 规格：
200Tests
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文献和实验Western Blotting with Alkaline Phosp
with phosphate-buffered saline (PBS).2.Aspirate excess PBS.3.Add 1ml boiling 2X concentrated electrophoresis sample buffer (125mM Tris pH 6.8, 4% SDS, 10% glycerol, 0.006% bromophenol blue, 1.8% beta-mercaptoethanol).4.Scrape cells from dish, transfer
Western Blotting with Alkaline Phosphatase Conjugates
-fold for the BCA (Pierce) protein concentration assay (SDS concentration must be below 0.1% to avoid interference with the colorimetric reading). For Protein Gel Electrophoresis of Cell Culture (without determining protein concentration)
Western Blotting with Horseradish Pe
must be below 0.1% to avoid interference with the colorimetric reading). For Protein Gel Electrophoresis of Cell Culture (without determining protein concentration) 1.Decant medium from 10cm dish of adherent cells and rinse plate rapidly with phosphate-buffered saline (PBS
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