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文献和实验flasc.15、add aminopterin; distribute on 96-wells cell culture plates (Nunc) (200µl/well); place in incubator (37℃, pCO2 7%) enveloped in aluminium paper.16、check the cultures 1-2-7 days after the fusion.17、on day 7-10 change the culture medium.18、test
Fusion is an important procedure to produce monoclonal antibodies (MoAb).
out from the number of spleen cells the fusion was started from); let to stay in incubator, 37ѓC, pCO2 7% for 2 hours in a big flasc. add aminopterin; distribute on 96-wells cell culture plates (Nunc) (200µl/well); place in incubator (37ѓC, pCO
monoclonal antibody production fusion
/ml (calculation figured out from the number of spleen cells the fusion was started from); let to stay in incubator, 37ѓC, pCO2 7% for 2 hours in a big flasc. add aminopterin; distribute on 96-wells cell culture plates (Nunc) (200µl
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