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- 详细信息
- 文献和实验
- 技术资料
- 英文名:
详见说明书
- 供应商:
广州华韵生物科技有限公司,www.huayunbio.cn
- 规格:
50次
从<100mg粪便样品中提取微生物RNA
产品简介:
HiPure Stool RNA Kit适合于从粪便样品在裂解液中匀浆,高温水浴进一步裂解,RNA释放到裂解液中。氯仿抽提去除基因组DNA和杂质,转移上清液加入无醇结合液,过柱子纯化RNA,最后RNA被RNase Free Water洗脱。洗脱的RNA可直接用于PCR、Southern 杂交、酶切等实验。
| 产品特性与优点: 纯度高 - 独特的吸附剂更高效去除抑制因子 高浓度- 能最大提取粪便样品中的总RNA 灵敏 -可处理低至pg级的RNA |
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文献和实验Stool DNA Protocol (for pathogen detection)
capable of at least 13,000 x g 2. Nuclease-free 1.5 mL or 2 mL microfuge tubes 3. Water bath or heating block preset to 65°C and 70°C 实验步骤 1. Weigh up to 50-100 mg of stool sample in a 2 mL centrifuge tube containing 200
Purification of DNA/RNA in a Microfluidic Device
Often, modern diagnostic techniques require the isolation and purification of nucleic acids directly from patient samples such as blood or stool. Many diagnostic tests are being miniaturized onto micro-sized platforms and integrated
4. Banerjee, H. and R. Singh, A simple crosslinking method, CLAMP, to map the sites of RNA-contacting domains within a protein. Methods Mol Biol, 2008. 488: p. 181-90. 5. Jensen, K.B. and R.B. Darnell, CLIP: crosslinking and immunoprecipitation of in vivo
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