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- 详细信息
- 文献和实验
- 技术资料
- 库存:
99
- 供应商:
攸碧艾生物
- 检测范围:
咨询
- 检测方法:
双抗夹心法
- 应用:
ELISA
- 适应物种:
咨询
- 标记物:
咨询
- 样本:
血清、血浆、组织匀浆、细胞培养物上清及其他生物体液
- 灵敏度:
咨询
- 规格:
48T/96T
| 规格: | 48T | 产品价格: | ¥1312.8 |
|---|---|---|---|
| 规格: | 96T | 产品价格: | ¥1745.4 |
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文献和实验The indirect ELISA is used primarily to determine the strength and/or amount of antibody response in a sample, whether it is from the serum of an immunized animal or the cell supernatant from growing hybridoma clones. Procedure 1. All incubations
, dry 5. block half of the plate (with platelets) by PBS-BSA 1% and coat another half by glycine (x1) - BSA for 1 hour at 37ѓC 6. wash 3 times in PBS-tween 20, dry 7. incubated at 37ѓc for 1 hour with a 1:1000 dilution of rat IgG2a anti-mouse kappa
PBS-PF (paraformaldehyde), 2% PBS-tween 20, 0.05% glycine (x1) - BSA, 1 mg/ml revelation solution (citrate/phosphate buffer), pH 5, 1 L, water solution = citrique acid, 5.1065 g + Na2 HPO4 x 2H2 0, 9.08 g
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