Anti-CDK2 (phospho T160) + CDK

Using Phospho‐Motif Antibodies to Determine Kinase Substrates

comprising both the phosphorylated residue and the surrounding residues that determine kinase specificity, with degenerate residues taking up the remaining positions. Currently, several categories of phospho?motif antibody are commercially available

【求助】抗体制备服务

largely independent of the surrounding amino-acid sequence. The antibody is phospho-specific but does not cross-react with phospho-serine-containing sequences. The anti-phosphothreonine monoclonal antibody is labeled with Eu3+ Cryptate by Cisbio

浅谈流式核内蛋白染色步骤

治”的颗粒——待测物复合体所散发的荧光，从而测定待测物的数量。详细原理介绍：每个CBA微球大小一致，具有特定的荧光强度，包被有适用于特定分析（如其它抗体或者可溶性蛋白）的特异性捕获抗体（Capture Antibody），提供了类似ELISA孔板的捕获表面。当微球和待测样品溶液混合后，微球上的特异性抗体就与样品（血清、血浆或者细胞培养液）中相应的抗原或蛋白结合，然后加入荧光标记的检测抗体，就会形成“三明治”夹心复合物。最后通过流式细胞仪对特异性目的蛋白进行检测。CBA的每种微球携带有不同强度的红色