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文献和实验Wholemount in situ hybridisation
. 5 Preblock the embryos with 10% sheep serum, 2% BSA in TBTX for 2-3 hours at room temperature. 6 Remove the 10% sheep serum, 2% BSA from the embryos and replace with the preabsorbed antibody (see below). Rock overnight at 4°C.
ANALYSIS OF PROTEINS BY IMMUNOPRECIPITATION
strongly to IgG from cow, goat, sheep, cow, horse, rabbit and guinea pig and to mouse IgG1 and IgG3 . Protein G can also bind bovine serum albumin (BSA). Thus, BSA should be added to buffers used with Protein G. Alternatively, recombinant Protein G
The In Situ PCR:Amplification and Detection in a Cellular Context
disease in sheep. Upon infection with these agents, the provirus frequently integrates into the host genome and establishes a persistent infection, whereby the infected cells are characterized by, among other things, a transcriptionally-quiescent state
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